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大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

阅读次数:371   发布时间:2022/7/29 16:51:13

产品名称:大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

产品规格:48T                 96T

 

厂家品牌;杭州齐誉生物科技有限公司

发货地:杭州

保质期:6个月

存放要求:2-8°避光防潮保存

1. 实验操作过程中需要自备物品:

2. 酶标仪(450nm

3. 高精度加样器及枪头:0.5-10uL2-20uL20-200uL200-1000uL

37℃恒温箱。

大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒 大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

温馨提示*杭州齐誉生物科技有限公司所提供的试剂盒均为科研用品,不得用于临床诊断使用。

本公司与各大,学校,科研所,取得了良好的合作。合作单位有:华南农业大学,华东理工大学,福建农林大学,武汉理工大学,汕头大学,中南大学,贵州大学,南华大学,苏州大学,江苏科技大学,安徽大学,四川大学,青岛大学,天津大学,西北农林科技大学,南京师范大学,西北医科大学,河南大学,上海曙光,医学科学院,上海华山,宁夏人民,香港大学等等。

欢迎新老客户来选购。

FOR RESEARCH USE ONLY. 

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

特殊服务:提供免费代测服务

杭州齐誉生物科技有限公司与各大高校、、科研单位等机构有合作关系。常年提供科研检测酶免ELISA试剂盒、生化试剂、常用耗材等。ELISA试剂盒种属包括:人类ELISA试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、豚鼠ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、猪ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、猴ELISA试剂盒、植物ELISA试剂盒等。

需要大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

样品类型:样本必须为液体,不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。1ml的全血可得到0.5ml的血清或血浆。每个标本量收集体积=100ul× 检测种类。取材向销售人员索要说明书。

样品采集:收集样本必须清楚要检测的成分是否足够稳定。对收集后当天进行检测的标本,存储在4°C备用。如有特殊原因需要周期收集标本,将标本及时分装后放在-20°C-70°C条件下储存。避免反复冻融。

注意事项:

  1. 试剂盒应在室温平衡15-30分钟后使用,酶标包被板开封未用完板条装入密封袋保存。

  2. 洗涤液出现结晶,稀释时可在水浴中加温,不影响结果。

    3请严格按照说明书操作,结果必须从酶标仪读数为准

    大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

  3. ELISA试剂盒是固相夹心法酶联免疫吸附实验(ELISA).已知待测物质浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将待测物质和生物素标记的抗体同时温育。洗涤后,加入亲和素标记过的HRP。再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用。产生颜色。颜色的深浅和样品中待测物质的浓度呈比例关系。

    分析类型:夹心ELISA

    样本类型:血清、血浆、组织、尿液、及相关液体等样本

    产品型式48/96孔板

    贮存方法:试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

    运输条件4℃

    ELISA酶联免疫试剂盒有(牛、马、山羊、绵羊、人、狗、兔、鸡、豚鼠、猪、植物、猴子、大鼠、小鼠、鱼,虾蟹,菌类等)种属相关试剂盒,

    本着客户至上的原则为客户提供的产品,公司产品有任何质量问题免费包退换(非人为原因导致)。同时,齐誉生物提供免费代测服务并确保数据的稳定性。大鼠细胞色素P4502E1(CYP2E1)elisa试剂盒视屏操作

    FOR RESEARCH USE ONLY. 

    NOT FOR USE IN DIAGNOSTIC PROCEDURES.

     

    Human S-adenosyl Methionine (SAM) ELISA Kit instruction

     

    Intended use

    This SAM ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The S Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SAM in the sample, this SAM ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SAM concentration. The concentration of SAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Sample collection and storages

    Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles

    Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.

    Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.

    Note:  The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.

    Materials required but not supplied

    1.  Standard microplate reader(450nm)

    2.  Precision pipettes and Disposable pipette tips.

    3.  37 ℃ incubator

    Precautions

    1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.

    2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.

    3.  Mix all reagents before using.

    Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)

    Materials supplied

    Name

    96 determinations

    48 determinations

    Microelisa stripplate

    12*8strips

    12*4strips

    Standard

    0.3ml*6tubes

    0.3ml*6tubes

    Sample Diluent

    6.0ml

    3.0ml

    HRP-Conjugate reagent

    10.0ml

    5.0ml

    20X Wash solution

    25ml

    15ml

    Chromogen Solution A

    6.0ml

    3.0ml

    Chromogen Solution B

    6.0ml

    3.0ml

    S Solution

    6.0ml

    3.0ml

    Closure plate membrane

    2

    2

    User manual

    1

    1

    Sealed bags

    1

    1

    Note: Standard (S0 S5) concentration was followed by:0,1,2,4,8,16 ng/mL

    Reagent preparation

    20×wash solution:Dilute with Distilled or deionized water 1:20.

    Assay procedure

    1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

    2.  Set Standard wells and testing sample wells. Add 5l of Standards or Samples to the appropriate well of the Microtiter Plate.

    3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesnt add anyting.

    4.  Add 10l of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 

    5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.

    6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

    7.  Add 50μl S Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not

    appear uniform, gently tap the plate to ensure thorough mixing.

    8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

    Calculation of results

    This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the erage O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.

    First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

    To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.

    Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.

    The sensitivity by this assay is 0.1 ng/mL

    Standard curve

     

     

    Storage  2-8.

    validity six months. 

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

     


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主营产品:生化试剂盒、生理生化检测、高效液相色谱检测、 气相色谱检测、Elisa试剂盒